Composite

Part:BBa_K1326002:Design

Designed by: Leah Simmons   Group: iGEM14_Macquarie_Australia   (2014-10-08)


Plac + CTH1 + YCF54 + Plasto + ChlM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BglII site found at 621
    Illegal BglII site found at 2409
    Illegal BamHI site found at 1476
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2966
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1638
    Illegal BsaI.rc site found at 311
    Illegal BsaI.rc site found at 467
    Illegal BsaI.rc site found at 1088
    Illegal BsaI.rc site found at 1947
    Illegal BsaI.rc site found at 2070


Design Notes

Codon optimisation and attachment of BioBrick prefix and suffix.


Source

Chlamydomonas reinhardtii

References